Generator

Part:BBa_K1741011:Design

Designed by: Marta Żardecka   Group: iGEM15_UAM_Poznan   (2015-09-17)


sfGFP under T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 48
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 48
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 48
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 131


Design Notes

None.


Source

All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.

References