Generator
Part:BBa_K1741011:Design
Designed by: Marta Żardecka Group: iGEM15_UAM_Poznan (2015-09-17)
sfGFP under T7 promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 48
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 48
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 48
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 131
Design Notes
None.
Source
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.